p100BP Open Source DNA Ladder Plasmid

Make your own DNA gel ladders for ultra cheap!

A convenient ladder-making plasmid from the legendary, but now defunct, Gene and Cell Technologies company. Fully open source and tested. Just miniprep and cut with EcoR1 enzyme to produce DNA fragments of known length up to and including 1000bp, in 100bp increments. Be sure to make as big of a miniprep as you can; at least 100ng/uL for a visible banding pattern upon digest. Image below is 17uL of 100ng/uL miniprep cut for one hour using EcoR1-HF (NEB). I used the NEB 1KB Plus ladder as reference (lane 1), 1% agarose TAE gel, and GelGreen (biotium) DNA stain.

Gel order:
NEB 1KB Plus

p100BP cut with EcoRI

p100bp uncut supercoiled plasmid

Please refer to my Plasmid Library for the sequence data of this plasmid.

For a protocol on how to use this plasmid, please see my Protocols Library for step-by-step instructions.

For the latest out of my research projects and hints at potential new products, check out my Open Lab Notebook, where all my work lives.

The plasmid is shipped pre-transformed in a safe non-pathogenic laboratory line of E. coli in an agar stab. Please store at 4C in a fridge or at room temp in the dark. DO NOT FREEZE. Properly stored unopened agar stabs have lasted well over 5 years with good recovery in my lab.

To maintain the plasmid in the E. coli it comes in, please use standard LB Agar with 100mg/L Ampicillin and incubate overnight at 37C. Please restreak when you would like to use the cells and only work with freshly grown well isolated colonies. Do not grow cells in liquid media directly from an agar stab as it will carry over stressed cells and lead to uneven colony sizes and poor growth performance.

pI100BP © 2024 by Sebastian S. Cocioba is licensed under Creative Commons Attribution-ShareAlike 4.0 International

LIABILITY WAIVER

By purchasing products from New York Botanics, LLC dba "Sebastian's Biotech Bazaar", henceforth referred to as the "Seller", you, the "Purchaser", agree to the following terms:

1. Acknowledgment of Risk: You acknowledge that the purchase and use of the plasmids involve known and unanticipated risks, which could result in physical or chemical damage, and you understand such potential risks.

2. Assumption of Risk: You knowingly, voluntarily, and expressly waive any claim you may have against the Seller for any such damages sustained as a result of purchasing or using the plasmids.

3. Release and Waiver: You hereby voluntarily release, forever discharge, and agree to indemnify and hold harmless the Seller from any and all claims, demands, or causes of action which are in any way connected with your purchase or use of the plasmids.

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By purchasing this product, you acknowledge that you have read this release of liability and assumption of risk agreement, fully understand its terms, understand that you have given up substantial rights by agreeing to it, and agree to it freely and voluntarily without any inducement.