Monthly Archives: March 2014

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Intro to Plant Tissue Culture: Preamble

Since this blog will be dedicated to everything plant biotech related, I thought I might start with an introduction to the most important aspect of plant biotechnology: Plant Tissue Culture. This will be a multi-part series where each individual post will be dedicated to one key aspect of plant tissue culture or PTC for short. Some of the topics to be covered in this series are (in no particular order) as follows:


  • History
  • Aseptic Technique
  • Basic Plant Biology
  • Traditional Plant Propagation
  • Micropropagation
  • Hormones
  • Troubleshooting
  • Antibiotics
  • Media
  • Sterilization
  • Callus Stage Culture
  • Shooting Stage Culture
  • Rooting Stage Culture
  • Advanced Plant Biology
  • Contamination
  • Acclimatization


These are all just a number of important topics that form the fundamentals of PTC and will suffice in developing a background understanding of the techniques and concepts used. I will continue to add more parts to this series so check the lectures section often for new posts. I see PTC and plant biotech in general as cooking rather than a stringent science. There are many things that need an instinctive response to ensure the optimal result and these instincts are fostered with time. I would like to divulge some of the lesser known tips and tricks which may help increase the desired results in your cultures. In the end, you need to practice, mess up, and try again in order to truly learn the skill but hopefully this series will help you avoid the myriad pitfalls I’ve fallen into when I first began practicing the dark-green arts. I hope you will enjoy this lecture series and learn something new and/or useful that can help you on your journey through the fascinating world of plant biotechnology. Yay, science!